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Why we use caffeine for HPLC calibration in Detector wave
ength accuracy test
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Answer Posted / suryabhan datir
caffeiene having different wavelents it having 2 maximas
and 1 minima.
1st maxima is 205nm
2nd maxima is 273nm
minima is 245nm
and it is primary reference standard and also suggested in
pharmacopiea.It is easily soluble in water. It shows
complete absorbance at multiwavelength
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In HPLC calibration, caffeine is used as primary standard for wave length calibration due to caffeine is having dual maxima at 273 & 205 nm and one minima at 245 nm. Any body can give reference of these details from any pharmacopeia (with chapter no.) or any other authentic guideline?
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