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Answer Posted / swapna
Sauton's medium is not available commmercially, it must be
prepared in-house.
Ingredients:
KH2PO4 0.5 g
MgSO4.7H2O 0.5 g
Citric acid 2.0 g
Ferric ammonium citrate 0.05 g
Glycerol 60 ml
Asparagine 4.0 g
Distilled water to 1000 ml.
Dissolve the reagents, except for the glycerol, in 300 ml
water using heat. Once dissolved, add the glycerol and make
up to 1 liter with distilled water.
Check pH and, if necessary, adjust to pH7.4 with 1 M NaOH.
Autoclave at 121 oC for 15 min.
Addition of ZnSO4 (0.1 ml of 1% solution) and Triton WR1339
(5 ml of 5% solution) improves growth of M. tuberculosis.
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