name the matrix used in hydrophobic interaction
chromatography?????
Answer Posted / balu
silica columns, Silica beads coupled with different lengths
of alkyl side chain are used for appropriate purpose.
C4 column -shorter alkyl chain (Larger proteins supposed to
have more hydrophobicity and therefore the chains with
shorter length have high chance of interaction. So For
larger proteins are separated based on their proportion of
protein hydrophocity)
C8 and C18- Longer alkyl chain (Smaller proteins or
peptides supposed to have less hydrophobicity and therefore
the chains with longer length have high chance of
interaction. So For smaller proteins or peptides separated
based on their proportion of protein hydrophocity)
| Is This Answer Correct ? | 3 Yes | 0 No |
Post New Answer View All Answers
What are catalysts?
Define ionic product of water. What is its value?
What does the expression –dc/dt indicates?
Explain who proposed law of mass action?
Define activation energy?
Explain which branch of science deals with the transformation of chemical energy into electrical energy and vice versa?
why we use 96 well plate in ELISA plate reader?
How many elements are there in 3d-series of first transition series?
give the co-ordination number of nacl?
What is galvanic cell?
To what organic group do pentoses belong?
Which element in 1A group does not form peroxides?
Which form of isomers of a substance is more stable?
Are nucleotides formed of only one type of pentose?
What is chemical formula for borax?