Interested to Buy Any Domain ? << Click Here >> for more details...
what is the general mechanism of high performance liqiud
chromatography?
- 7 Answers
- 18518 Views
- Ajanta Pharma, Amneal Pharmaceuticals, I also Faced
- E-Mail Answers
Answer Posted / nabil
Confusigly,there are two variants in use in HPLC depending
on the relative polarity of the solvent and the stationary
phase.
1-Normal Phase HPLC
The column is with tiny silica particles, and the solvent
is non polar hexane for example, Polar cpds in the mix
being passed through the column will stick longer to the
polar silica than the non polar cpds will, the non polar
ones will therefore pass more quickly through the column.
2-Reversed phase HPLC
In this case the silica is modified to make it non polar by
attacheing hydrocarbon chains to its surface typically with
either 8 or 18 carbon atomsin them. A polar solvent is used.
In this case there will bestrong attraction between polar
solvents and polar molecules will pass through
thecolumntherewon't be much attractoin between the
hydrocarbon chain and the polar solvent.
Non polar cpds in the mix will tend to form attractions
with the hydrocarbons gps because of van der waals forces
they will be also less soluble in the solvent because the
need to break the hydrogen bonds,they spend less time in
solution in the solvent, that mean the polar molecules that
will travel through the column more quickly.
| Is This Answer Correct ? | 4 Yes | 2 No |
Post New Answer View All Answers
For limit test of heavy metals in BP, Method C require that the substance is ignited at a temperature not exceeding 800 °C. Why confines such the temperature?
what is turbidimetric titration?Give 2 examples.
what is diffrence between specificity and selecivity?
how you confirm the assay method?
Why we use potassium dichromate in uv calibration Exact reason behind it??
what is the principle of UV Vis spectroscopy, AAS, ICP OES,ICPAES, ICP-MS and FTIR
How do we get end points and how many end points are possible for citric acid and di-acid not theorotically answer should be given practically.
Why only Copper standard is used to calibrate Atomic Absorption spectrophotometer?
how the compound separate in coulunm,explain
we can use expired sample for validation and analyst qualification?
what is the origin to prepare standard operating procedure
process of Diclofenac sodium,IP.
what is biorelivent dissolution media?
What is the formula to determine the concentration of M of a solution given the % transmittance? Use %T = 43.7 as an example.
Which products will produce when acetic anhydride reacts with Ter-butanol, Sec-butanol, Iso butanol, Dimethylamine etc.
