If compound Pka is 5 then which buffer you will select ?
Answer Posted / shyam kumar
The thing is u should not use mobile phase pH 5.00. any pH
u can use generally +/- 1.00 unit is preferable. in that
also selection of lowe or higher pH is depending on your
compound nature and noise. in whis pH u have less noise
choose that.
| Is This Answer Correct ? | 19 Yes | 0 No |
Post New Answer View All Answers
Loss of linearity in tkn analysis, possible reasons?
why we use a particular hplc column for a particular compound give reasons?please
identification is for unknown? qualification for known? reporting for LOQ?
how to get accurate result for Residue on evaporation in purified water
In which situation we require to analytical method validation of excipient?
what is mean by 40 in the dissolution basket mesh size?
what is %labelled amount in content uniformity of dosage unit and its calculation?
What is the principle of Thermal conductivity detector and FID?
How to know the estimated LOQ concentration in ppm
if identification threshold crosses the limits then what next step?
please explain about aluminium hydroxide assay
2. Two grams of Benzoic acid are dissolved in 200 ml of water and extracted with 200 ml of diethyl ether. The distribution coefficient of benzoic acid is 100, and its dissociation constant is 6.5 10-5. Calculate the distribution ratio (D) of benzoic acid at pH 2, 5, and 6. 3. Calculate D at pH 2 to 10 (1 unit apart) in the above problem, and plot D versus pH.
WHAT IS THE PARAMETER FOR THE SELECTION OF MOBILE PHASE IN HPLC?
What is a difference between potency and purity?
what is mean by dissolution biowaiver study?