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Answer Posted / hemant patel
Column chromatography is a separation technique in which the
stationary bed is within a tube. The particles of the solid
stationary phase or the support coated with a liquid
stationary phase may fill the whole inside volume of the
tube (packed column) or be concentrated on or along the
inside tube wall leaving an open, unrestricted path for the
mobile phase in the middle part of the tube (open tubular
column). Differences in rates of movement through the medium
are calculated to different retention times of the sample
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