Answer Posted / sandeep negi

in the above procedure posted by me
mr. sandeep negi
i want 2 say
2 important points 2 remember
1) our master is (4.5ml saline+.5ml sample) i.e 1:10
dilution
2)from 2nd row till 7th tube sample is diluted
and at the 7th tube of all the row
1ml =(.5ml saline+6th diluted vol .5ml )
and after mixing properly .5ml is discarded

**in negative control tube i.e 8th tube only .5ml saline
+.5ml antigen is added

hope all of u will be satisfied by me
thanks

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