Generally for Normal Phase HPLC,stationary phase is Polar.In
place of polar if we take non-polar means what happened & why?

Answer Posted / umesh patel

in case of normal phase chromatograpy, as we are using
highly non polar sovent as mobile phase ,it will dissolve
the column bed of non polar SP

like dissolves like

Is This Answer Correct ?    7 Yes 3 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

what is the extinction coefficient for Indomethacin or at 319nm wave length.

3512


How to calculation of residual solvent methanol (3000 ppm) standard solution, and how many quantity used of test sample

2520


why require to add enzymes in capsules dissolution only? is it require to tablet also?

256


How to set specification of assay, fisdolution and related substances?

354


if your product is soluble in 0.1n hcl and water then which you choose as media from these 2 media?

269






How require to develop GC method? how to select diluent, GAS, column selection and other chromatographic conditions?

267


what is the purge flow & how to calculate

1968


what is the principle involved in turbidometry and nephelometry ?

3626


before starting analytical method valodation what you checking? and how giving preference to start validation?

331


what is the structure of DOWEX-50

4912


How they found 1mL of K.F reagent is equivalent to 5mg of water and if we change the composition of K.F reagent, is it can neutralize more amount of water?

1293


cefoperozone and sulbactam inj. hplc test method

2117


how to set sample and standard concentration in RS method?

354


Why acetonitrile and water are used as extraction solvent when analysis melamine? I thought they are miscible and won't be able to separate...

1210


what is procedure of actual procedure for registration of drug?

1446