What is colimn selection Criteria in HPLC analysis?
Answer Posted / m
Column selection is the most important part of your HPLC
method development
1 Choose the column according to the your column polarity
and pH range of buffer.
2 Mobile phase composition and what solvent you are used is
also depends
3 Carbon loading and surface area and micron size is also
depends if you are using more buffer concentraion (Aques)
for that hydro columns are also available
over all it depends on your compound and its polarity
| Is This Answer Correct ? | 21 Yes | 0 No |
Post New Answer View All Answers
in which situation require to change rs specification?
What is stray light in uv what are units for stray light
Qc interview questions
how to develop the icp ms method? Application of icp ms?
Ph meter can show more than 14 ph reading? Why ph range in between 1 to 14 only?
inhouse product is in capsule form in combination and RLD is in tablet form then can we proceed for multimedia CDP? in inhouse capsule product disso is paddle with sinker in release media is there then RLD product in tablet form then with same as paddle with sinker we can proceed n.a.?
CAN WE DETERMINE THE PEAK PURITY IN GAS CHROMATOGRAPHY ANALYSIS?
How to set specification of assay, fisdolution and related substances?
4. Describe the operation of the Craig apparatus. Chapter.3 Equilibrium Processes in Separations
for inorganic molecules require to do RS, Assay and disso?
what is mean by 40 in the dissolution basket mesh size?
Why are use silicon oil mr?
WHAT IS THE PARAMETER FOR THE SELECTION OF MOBILE PHASE IN HPLC?
Difference between hlaf and rlaf
how to qualify the impurity?