Answer Posted / m

Column selection is the most important part of your HPLC
method development

1 Choose the column according to the your column polarity
and pH range of buffer.

2 Mobile phase composition and what solvent you are used is
also depends

3 Carbon loading and surface area and micron size is also
depends if you are using more buffer concentraion (Aques)
for that hydro columns are also available

over all it depends on your compound and its polarity

What are the primary considerations in extractables and Leachables ?

2208

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how to qualify the impurity?

780

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What is the requirement for brazil in the analytical method vslidation comparr to ich?

694

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why salisylic acid not using now days for disdolution calibration?

710

---

what is lod and loq ?,why use k2cr2o7 , kcl h2so4 in uv calibration ?,why use benzophenone & caffene acetone in hplc calibration ?,what is leading peak in hplc ?why we do the calibration of limit of stry light in hplc & uv ?

3618

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USP methodology, EP methodology, IP methodology, among three if possible to use one methodology for qualify working standard to use USP, EP, IP ? Please explain...

1966

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in monograph 7 imp are there but in vendor showing 5 imp do not req to include 2 imp for that what will be justification?

663

---

what parameters we will consider while developing a HPLC method and how we confirm our HPLC method is valid?

3623

---

Difference between the quantitative analysis and qualitative analysis?

1102

---

How to fix the sample conc. in Related substances test by HPLC?

1025

---

What is control room temperature and which guide line says?

1578

---

what is the purge flow & how to calculate

2557

---

What is the use of tlc and hplc? And when and where use?

1093

---

if you have given one product then how you determine the impurity in that?

688

---

what is mean by covalidation

729

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