What is colimn selection Criteria in HPLC analysis?
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Column selection is the most important part of your HPLC
method development
1 Choose the column according to the your column polarity
and pH range of buffer.
2 Mobile phase composition and what solvent you are used is
also depends
3 Carbon loading and surface area and micron size is also
depends if you are using more buffer concentraion (Aques)
for that hydro columns are also available
over all it depends on your compound and its polarity
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My question about gas chromatography sulfur chemiluminsecence detector. I test unknown sample gas by GC-SCD (calibrated ) and the result of *H2S is 279 PPM , *but when I test the same sample with the GC-TCD (calibrated ) the value of *H2S is 2500 PPM . I'd like to inform you that both GCs are calibrated and have very good operation conditions with stable parameters . the question is if the sample gas with higher H2S over detection limits of SCD detector (1000 ppm). why the result it 279 ppm Best regards
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