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Answer Posted / sahitya
Individual ampules of virus-suspension are frozen and
stored at –76 C, then titrated under the exact conditions
of time, temperature and serum-concentration as employed in
the neutralization-test. Based on the determined range of
variability of host-susceptibility to each virus, the
higher dilution for the neutralization-test was selected,
thus obtaining maximal sensitivity. The value of the
function of this dilution to the fifty per cent end-point
dilution is adopted as a "actor" for use with any other
titrated suspension of the same virus.
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