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Why caffeine as a reference standard used for calibration
of HPLC?
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Answer Posted / yojana
it is use as primary standard .it gives two maxima & one
minima
first maxima at 205 nm
second maxima at 273 nm
&
minima at 245nm
also caffeine easily available in market.
| Is This Answer Correct ? | 37 Yes | 7 No |
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In HPLC calibration, caffeine is used as primary standard for wave length calibration due to caffeine is having dual maxima at 273 & 205 nm and one minima at 245 nm. Any body can give reference of these details from any pharmacopeia (with chapter no.) or any other authentic guideline?
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