Interested to Buy Any Domain ? << Click Here >> for more details...
Answer Posted / bricy
The invention provides a method for the purification of a
virus from a host cell, the method comprising the steps of:
a) culturing host cells, b) infecting the host cells with a
virus, c) treating the cell culture with nuclease, d)
lysing the host cells to provide a lysate comprising the
virus. The virus is preferably a recombinant adenovirus.
The invention further provides a method for the
purification of a recombinant virus expressing a
heterologous protein that is capable of binding nucleic
acid, comprising the steps of: a) culturing host cells, b)
infecting the host cells with the recombinant virus, c)
lysing the host cells to provide a lysate comprising the
recombinant virus, d) subjecting the recombinant virus to
anion exchange chromatography and size exclusion
chromatography, characterized in that the virus-containing
mixture is buffer exchanged at least once with a solution
comprising at least 2 M NaCl, or another salt providing an
equivalent ionic strength. (end of abstract)
| Is This Answer Correct ? | 0 Yes | 0 No |
Post New Answer View All Answers
Explain the difference between an acid and a base?
I have complete my master in biotechnology and i got 59%... so what to do ahead ? i want to job somewhere but i am aunable to search it.. can anyone help me to find a well-known company where i can take a training or can do the job ? can I join any classes ??
Explain the major organs of the digestive system do?
What does boiling do to an enzyme?
Define telophase?
How erlenmeyer flask?
Why mosquito bites and it causes itching?
Explain what does an anther have on it?
Explain the difference between living and non-living things?
What is concentration gradient?
How do environmental factors affect growth ?
How many different cranial nerves are there in the human body?
What reflex causes the extension of the big toe and fanning of the other toes when the bottom of the foot is stroked?
What is the classification that divides orders?
What are the lowest and highest temperatures humans can survive?
