how rotenone acts as an inhibitor of electron transport?
Answer Posted / mithilesh
It is exracted from rots of derris elliptica and
lonchoncarpus nicou.it complexes avidly with nadh
dehydrogenase and acts between fe-s proteins and
ubiquinones.only 30 nanomoles per gram of mitochondrial
protein are effective for blocking sites.
| Is This Answer Correct ? | 0 Yes | 1 No |
Post New Answer View All Answers
What is heat of combustion?
Name two compounds, which is mixture of lucas reagent?
Why nitrogen cannot form penta halides?
Do the phosphate and the pentose groups give homogeneity or heterogeneity to the nucleic acid chains? Supported by that which of those groups is expected to participate in the highly diverse and heterogeneous genetic coding, i.e., Which of those groups is the basis of the information for protein production?
What is the structure of cscl and give the co-ordination number of Cscl?
What are some examples of chemical and physical changes?
What are pentoses?
What is the shape of molecules SF6 and IF7?
Which enzymes in cows allow them to digest cellulose?
What are the main factors that alter the speed of enzymatic reactions?
What is activation energy?
What is meant by the 5’ and 3’ extremities of nucleic acids?
Concerning their biological function what is the difference between rna and dna?
To calculate the e.m.f of the cell when does the nelsons equation is use?
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line