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What is the diffrence between gram-positive and gram-
negative bacteria?
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Answer Posted / sushworld
i want to focus just on staining.
gram +ve are those which are stained by crstal violet
stain. the gram -ve are also stained by crystal violet but
this stain is removed by washing it with 95% alcohol.this
is because it has lipids in it's cell wall which causes
pores in it's wall & lets the stain to escape.
It is hence stained with a counter stain saffranin which
gives it a pink colour.
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