Answer Posted / francois niyonsaba

To my knowledge, it is said that 2-ME is important for
lymphocyte or other cell cultures because the amino acid
cysteine is in short supply in FBS used in culture media,
while there is plenty of cystine. For example, T cells are
unable to transport cystine into the cytosol, where it must
be converted into cysteine. 2-ME reduces cystine into a
form that can be transported by the cells and is then
converted to cysteine needed for cell growth.
Another reason is that 2-ME, as a reducing agent, can break
down many of the toxic metabolites produced by cells in
culture thus improving the environment around the cells.


Francois NIYONSABA, M.D., Ph.D.
Associate Professor
Atopy/Allergy Research Center
Juntendo University School of Medicine
2-1-1 Hongo, Bunkyo-Ku
Tokyo 113-8421 JAPAN
Tel: +81-3-5802-1591
Fax: +81-3-3813-5512
E-mail: francois@juntendo.ac.jp

Describe about penicilin streptomycin ?

1852

---

Is it possible for the transfection of primary neuronal cell cultures?

1787

---

what do you know about May-Grunwald -Giemsa staining?

2245

---

How to Measure nitrite from LPS-activated RAW264.7 cells ?

2341

---

What is autophagic intracellular digestion? Why is this type of intracellular digestion intensified in an organism undergoing starvation?

950

---

Which are the cell organelles that participate in the cell division and in the formation of cillia and flagella of some eukaryotic cells?

1003

---

Do bacteria cells have nucleus?

853

---

How to monitor gene expression over time in culture - GFP method ?

1810

---

what is macrophage suspension ?

1893

---

Explain the Procedure for Extraction of red blood cells?

1898

---

How is collagen measured?

1929

---

Is there any bacteria made of more than one cell?

909

---

Explain the PC12 transfection ?

1729

---

What are the main products of plasma cells called?

774

---

How to use Lidocaine for primary astrocyte culture purification ?

1775

---