Interested to Buy Any Domain ? << Click Here >> for more details...
Answer Posted / korokoro
My advisor suggest,
1. TE buffer 20 ul(in TE has Tris that preserve DNA) +
Phenol 5 ul + 1 colony
2. vortex mix about 2-3 min (or until cell lysate,
afterthat centrifuge at 12000 rpm 5 min or just spindown
3. run gel eletrophoresis compare with positive control and
negative control
Principle is lysated cell to check DNA band compare with
same strain bacteria that contain target plasmid and this
method i used to screen transformant()
(but... it's not work for me because positive and negative
control has same band)
Anyone have a rapid and simple method to screen plasmid in
transformant?
| Is This Answer Correct ? | 0 Yes | 2 No |
Post New Answer View All Answers
How does ELISA work?
What are Prions?Are they a new form of life?
Explain about the buoyancy of yeast in media ?
Explain the importance of species of the molds Ustilago and Claviceps in medicine.
what is the method for membrane separation?
Describe the Immunofluorescent staining of Actinomycetes?
What are the advantages of quaternary ammonium compounds?
Which are the theories of gram staining?
Why did you choose BSC with Microbiology after your diploma in pharmacy and than MBA ? You also could do B.pharmacy and MBA?
properties of fehling's reagent
What is quaternary ammonium salt give an example?
Explain the Soft top agar AND Ames test ?
What is RNA microinjection ?
Give an account on plasmid screeening?
Describe the infectious cycle of a negative strand RNA virus?
