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Answer Posted / deepa
The Widal test is made to diagnoise Typhoid fever.
procedure for widal tets is as follows.
1.Make a series of serum dilutions for each antigen to be
tested. Include tubes with 0.5 ml saline for control of
each antigen to be used.
2.Use perfectly clean and dry test tubes and prepare
dilutions beginning with 1:10 and doubling through 1:320 or
so.
3.Add 0.1 ml of serum to 0.9 ml of physiological saline and
then dilute serially by mixing 0.5 ml diluted serum with
0.5 ml saline and discarding 0.5 ml from the last tube.
4.From specimen submitted to detect possible rise in titre,
prepare a series of 10 dilutions, ending with 1:5120.
In this way Widal test is performed.
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