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Question 89 - A hemocytometer is a device that is used for counting cells. In an engineering experiment, 100 microlitres of cell suspension is diluted with 50 microlitres of Trypan blue dye. Only death cells appear blue in color when stained with the dye. There are 57 cells detected in a hemocytometer, where 5.3 % of them appear blue when the chamber of the meter is placed under a microscope. Each square of a chamber can contain 0.0001 mL of liquid. (a) Calculate the number of viable cells. (b) The cells ocupied 5 squares. Calculate the average number of viable cells / square. (c) Calculate the dilution factor of the cell suspension by using the formula : Dilution = final volume / initial volume. (d) Calculate the concentration of viable cells / mL by using the formula : Concentration = (Average number of viable cells / square) x dilution x (square / volume).

Question 90 - In the calculation of the growth of bacteria, absorbance, A in spectrophotometry is used. According to Beer-Lambert Law, A = e x l x c where A is the absorbance of the solution (no unit), l is the distance of light travels through the solution (in cm), e is the molar absorptivity or the molar extinction coefficient [ in L / (mol.cm) ]. For a particular solute and fixed path length : As / Ao = Cs / Co where Ao is the observed signal for a known concentration Co, and As is the observed signal for a sample concentration Cs. (a) For a cell concentration of 560 cells / mL, a spectrophotometre gives an absorbance reading of 1.0. A mixture of concentration 3600000 cells / mL can be diluted in several operations, with each operation having a dilution of 1:20. How many dilutions should be made so that the concentration of this mixture can be calculated within a range of A = 0.0 to 1.0. (b) In another experiment, a sample tube of 1 cm in width is used. Let A = 0.06 and e = 0.0012 ml / (cell.cm). Find the cell concentration of the sample.

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Why does ATM use the cell of small and fixed length ?

What are the advantages of cell switching that is used in ATM?

Discuss the following terms with respect to ATM: VPI, UNI, asynchronous, AAL, Cell, PVC.

What is the wave equation for the sound waves encountered in smart cell phones, specifically android?

UNIT OPERATION - EXAMPLE 9.1 : Which of the sequence below represent a feasible flows of ethanol processing plants using cellulose as starting material? A. raw material -> heat exchanger -> distillation column -> reactor. B. reactor -> distillation column -> raw material -> heat exchanger. C. heat exchanger -> raw material -> distillation column -> reactor. D. raw material -> heat exchanger -> reactor -> distillation column. E. distillation column -> raw material -> reactor -> heat exchanger.

ENGINEERING MATERIAL - EXAMPLE 12.3 : Let a ^ 2 = a x a and a ^ 3 = a x a x a where ^ is power function. Niobium is a metal with a body-centered cubic structure. The length of the unit cell structure is b = 0.3349 nm. (a) Find the volume for a unit cell structure for niobium. (b) There are 2 atoms per unit cell structure of niobium. The metal has a molar mass of 92.9 g / mol. One mole of the metal consists of 6.02 x 10 ^ 23 atoms. Find the mass of niobium per unit cell and the density of niobium.

BIOPROCESS ENGINEERING - EXAMPLE 14.1 : In differential centrifugation of cells with diameter D in centimeter, the square of D is given by D x D = [18n ln (RF / RI) ] / [ (RP - RFF) Wt ] where n is the fluid viscosity (poise), RF is the final radius of rotation (cm), RI is the initial radius of rotation (cm), RP is cell density (g / ml), RFF is the fluid density (g/ml), W the square for the rotational velocity in (radians / s) (radians / s), t is the time required to sediment from RI to RF (s). Derive an equation for W as a function for D, n, RF, RI, RP, RFF and t, with the stated units above, in radian & degree.

Hadoop Libraries and Utilities and Miscellaneous Hadoop Applications?

MICROBIOLOGICAL ENGINEERING - QUESTION 28.2 : A hemocytometer is a device that is used for counting cells. In an engineering experiment, 100 microlitres of cell suspension is diluted with 50 microlitres of Trypan blue dye. Only death cells appear blue in color when stained with the dye. There are 57 cells detected in a hemocytometer, where 5.3 % of them appear blue when the chamber of the meter is placed under a microscope. Each square of a chamber can contain 0.0001 mL of liquid. (a) Calculate the number of viable cells. (b) The cells occupied 5 squares. Calculate the average number of viable cells / square. (c) Calculate the dilution factor of the cell suspension by using the formula : Dilution = final volume / initial volume. (d) Calculate the concentration of viable cells / mL by using the formula : Concentration = (Average number of viable cells / square) x dilution x (square / volume).

MICROBIOLOGICAL ENGINEERING - QUESTION 28.3 : In the calculation of the growth of bacteria, absorbance, A in spectrophotometry is used. According to Beer-Lambert Law, A = e x l x c where A is the absorbance of the solution (no unit), l is the distance of light travels through the solution (in cm), e is the molar absorptivity or the molar extinction coefficient [ in L / (mol.cm) ]. For a particular solute and fixed path length : As / Ao = Cs / Co where Ao is the observed signal for a known concentration Co, and As is the observed signal for a sample concentration Cs. (a) For a cell concentration of 560 cells / mL, a spectrophotometre gives an absorbance reading of 1.0. A mixture of concentration 3600000 cells / mL can be diluted in several operations, with each operation having a dilution of 1:20. How many dilutions should be made so that the concentration of this mixture can be calculated within a range of A = 0.0 to 1.0. (b) In another experiment, a sample tube of 1 cm in width is used. Let A = 0.06 and e = 0.0012 ml / (cell.cm). Find the cell concentration of the sample.