Answer / Raviendra Maurya

To prepare 80%, 100%, and 120% recovery solutions, you would need to spike your known impurity into a solvent (usually the same as the sample) to achieve the desired concentration. Here's how:

1. Calculate the amount of impurity needed for 100% solution: If the known impurity weight is I (in mg), then the amount needed for 100% solution would be 1mg (since the sample concentration is 1mg/ml).
2. Prepare 100% solution and dilute to prepare other solutions:
- For an 80% solution, take 80% of the 100% solution and dilute it with the solvent to make up to 100 ml.
- For a 100% solution (which you already have), dilute it with the solvent to make up to 100 ml.
- For a 120% solution, take 120% of the 100% solution and dilute it with the solvent to make up to 100 ml.
3. Always ensure that the total impurity concentration in all solutions does not exceed the known limit (NMT0.1%) to stay within acceptable limits.

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