BIOCHEMICAL ENGINEERING INSTRUMENTATION - EXAMPLE 29.3 : In thin layer chromatography (TLC), let retention factor Rf = (distance traveled by solute) / (distance traveled by solvent). Silica gel is used as stationary phase which is more polar than the hexane solvent as mobile phase. If the Rf values of 3 solutes A, B and C are 1.5, 0.75 and 1 respectively, compare the polarities of A, B and C with reasons.

Answer Posted / kangchuentat

BIOCHEMICAL ENGINEERING INSTRUMENTATION - ANSWER 29.3 : Polarities : A < C < B. A is least polar since it travels farthest with the mobile phase, whereas B is most polar as it is attracted to the stationary phase and travels least. The answer is given by Kang Chuen Tat; PO Box 6263, Dandenong, Victoria VIC 3175, Australia; SMS +61405421706; chuentat@hotmail.com; http://kangchuentat.wordpress.com.

Is This Answer Correct ?    0 Yes 0 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

What is the method adopted to minimize shell side pressure drop in a shell?

610


Are you having any experience in antibody designing?

2024


Plz if anybody has placement paper of HAL mail me i m Chemical engg my id er.kotnala@gmail.com

2031


What steps can be taken to avoid stress corrosion cracking (SCC) in steel vessels used for storing anhydrous ammonia?

1704


Do you have any idea on recombinant protein expression?

595






When an expansion is joint needed on the shell side of a shell and tube heat exchanger?

620


How to caliculate the boil up rate, dia and height in both packed and tray distillation columns and any referance books for the design.

7960


Explain the significance of the angle of repose holds in the chemical industry?

663


how did you spend today

1662


What are some common causes of gas pipeline vibration 20 carbon steel line?

705


Where barrel pump use?

1398


What is a good source of information for the design of pressure vessels?

582


why 14.7 pound/inch2 is used in idustries as unit of pressure bcz here not sea level and not a temperature is 0C (zero degree celcius)?????????

1686


Does home used kerosene which is available in blue color can change its color after keeping number of years (say 8-10 years)? If yes, then in which color will it turned to? Will it change to yellow color or transparent color? Please reply with suitable technical suggestion/approach. Its an important question which was asked. I need a very strong technical reply. Please help me.

1131


What is cathodic protection, how it works in Turbine condensers ?

2425