MICROBIOLOGICAL ENGINEERING - QUESTION 28.2 : A hemocytometer is a device that is used for counting cells. In an engineering experiment, 100 microlitres of cell suspension is diluted with 50 microlitres of Trypan blue dye. Only death cells appear blue in color when stained with the dye. There are 57 cells detected in a hemocytometer, where 5.3 % of them appear blue when the chamber of the meter is placed under a microscope. Each square of a chamber can contain 0.0001 mL of liquid. (a) Calculate the number of viable cells. (b) The cells occupied 5 squares. Calculate the average number of viable cells / square. (c) Calculate the dilution factor of the cell suspension by using the formula : Dilution = final volume / initial volume. (d) Calculate the concentration of viable cells / mL by using the formula : Concentration = (Average number of viable cells / square) x dilution x (square / volume).
Answer Posted / kangchuentat
MICROBIOLOGICAL ENGINEERING - ANSWER 28.2 : (a) Number of death cells = Total cells x % of death cells / 100 = 57 x 5.3 / 100 = 3.021 rounded to 3 cells. Number of viable cells = Total cells - number of death cells = 57 - 3 = 54 cells. (b) Average number of viable cells / square = 54 / 5 = 10.8 cells / square. (c) Dilution = final volume / initial volume = (100 + 50) microlitres / 100 microlitres = 1.5. (d) Concentration = (Average number of viable cells / square) x dilution x (square / volume) = 10.8 cells x 1.5 x (1 / 0.0001 mL) = 162000 cells / mL. The answer is given by Kang Chuen Tat; PO Box 6263, Dandenong, Victoria VIC 3175, Australia; SMS +61405421706; chuentat@hotmail.com; http://kangchuentat.wordpress.com.
Is This Answer Correct ? | 0 Yes | 0 No |
Post New Answer View All Answers
how much maximum power can be generated by 320v, 10kg-cm synchronus motor if shaft is roteted mechanically at 50 to 60 rpm?
What are the applications of screen analysis in the chemical industry?
what is the purpose of capacitor? and capacitor load means what? how does it connect?
have any one question paper of institute of plasma research center for aptitute as well as technical?
Explain what are some typical applications for glass-lined reactors?
What is the best way to control an oversized, horizontally oriented shell and tube steam heater?
Name some factors to consider when trying choosing between a dry screw compressor and an oil-flooded screw compressor?
The process conditions of the fuel gas are as follows: Temperature = 25 deg C Pressure = 4 bar g Calculate the line size for the fuel gas supply line to the superheater
Explain what is a quick way to calculate frictional pressure drops in carbon steel pipe?
What is the Import Procurement Cycle ? and what are the customization steps in SAP ?
How many years you are having experience in supervising others in lab environment?
What is maximum recommend pipe velocity for dry and wet gases?
How can you control the ph level in our cooling water with respect to ammonia contamination?
what is R1,R2,R3, R4........?
Define "good" cooling tower water?