Question { Intas, 77178 }

What is principle of Polarimeter?

Answer

Polarimetry measures the rotation of polarized light as it
passes through an optically active fluid. The measured
rotation can be used to calculate the value of solution
concentrations; especially substances such as sugars,
peptides and volatile oils. A polarimeter consists of a
polarized light source, an analyzer, a graduated circle to
measure the rotation angle, and sample tubes.

The polarized light passes through the sample tube and
exhibits angular rotation to the left (-) or right (+). On
the side opposite the polarizer is the analyzer. Using
optics, visual fields are manually adjusted by the user to
measure the optical rotation angle.

Polarimeters offer high accuracies where precision is
critical in determining the concentration of samples. Cole-
Parmer offers manual polarimeters where you look through a
viewing scope to read values on a vernier scale, and
semiautomatic polarimeters that have a digital display.
Polarimeters can measure in angle of rotation (¡),
International Sugar Scale (°Z), or both.