What was the primary antibody that was used?1 3949
Describe how you used that antibody to visualize β-gal protein?1 5122
Review your data from the entire module. Say we ask you to redo the purification.Is there any step that you could eliminate? Which one? Why can you eliminate it?2 3419
Why do proteins that recognize specific DNA sequences usually bind in the major groove of DNA?2 3935
An unusual structure is formed at the 5? end of each cellular mRNA. What is it? After this has formed, does the mRNA have a 5? terminus? Explain.1 2914
Splicing of pre-mRNA involves an unusual phosphodiester bond. Why is it unusual? What parts of the intron/exon form this bond?1 3487
Many genes are regulated in both a negative and a positive manner. The genes mediating lactose metabolism in bacteria are a classical example. What are the two small molecule ligands that control expression of these genes? What does each one do?1 3270
Some proteins profoundly alter the structure of DNA when they bind, bending it far out of its standard B configuration. Where do you think the energy comes from to distort the DNA?1 3488
Is there a biochemical test for maltose?2 17093
When starch reacts with iodine, why does it create the characteristic blue-black colour?5 18587
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