Un-Answered Questions { Sciences }

why didn't you go back to previous employer after shifting to bangalore?

1733


I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line

1718


what are advantages of excess populatoin?

2114


how to prepare for mp psc (botany)

3091


1 Briefly describe your ideal job: 2. What goals do you have in your career? 3. How do you plan to achieve these goals?

1886


I want to know about P.G courses &details

1672


If 2 is a prime no , then write a C program to test a number is prime or not

1545


How to prepare for the post State bank of india clerk personal interview ?

1952


mud eel eg.cuchia,how many gill rakers?

1725


i am preparing for Drug inspector exam.If u have previous questions please send me.

2216


NO OF PLANT IN ONGC

1846


i am mathematics student. i have selected on sbi cerck on20 oct.ple any one before me has exp of PI ple help me waht they ask in PI.and what about mathematics ques ple contact on shahkr786@gmail.com

1729


any one who has interviewed at noida for SBI clerk i have my interview call on 23.10.2008

1765


Please can someone tell me, how many times during a 12 hour period, do the hands of a clock face opposite each other? I believe its 11. Also, would this be the same, no matter WHAT the starting time? i.e. if the 12 hour period started at 6 o clock, would it still be 11 times? Many thanks for your help.

1765


bane the by products when crude oil is refined by distillation

1679