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 Micro Biology interview questions  Micro Biology Interview Questions (630)
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 Biological Sciences AllOther interview questions  Biological Sciences AllOther Interview Questions (35)
Question
if there is solution , how will u estimate that it contains 
DNA only, not RNA? how we differentiate DNA ,RNA and 
Protein in solution without studying its structure?
 Question Submitted By :: Bio-Technology
I also faced this Question!!     Answer Posted By  
 
Answer
# 1
Spectrophotometrically.OD at 260nm-DNA,280 for proteins 
Is This Answer Correct ?    44 Yes 5 No
Tk
 
Answer
# 2
alkaline hydrolysis -rna is hydrolysed and become less
viscous while dna has no change .. when we shake a tube
containing pure proteins,it frothes... also takin OD at
260nm is a good option for distinguishing between dna and
protein .

pliz mail me if there is any mistake in this answer. thanx.
 
Is This Answer Correct ?    21 Yes 3 No
Jyothi
 
 
 
Answer
# 3
just pour the solution in CsCl coloumn, and you will get
different bands. Also there is one very easy method to
detect protein solution from other two by just shaking. on
shaking solution containing protein will start foam
formation while those of DNA n RNA containing will not.
 
Is This Answer Correct ?    4 Yes 1 No
Abhinav Trivedi
 
Answer
# 4
value of od at240 / od at 260 = 0.8 for all nucleic acid. 
Is This Answer Correct ?    0 Yes 4 No
Aravind
 
Answer
# 5
staining methods for DNA and RNA run on gel for DNA and RNA
for protein acylamide gel
 
Is This Answer Correct ?    0 Yes 5 No
Anuj
 
Answer
# 6
use Nanodrop instrument 
Is This Answer Correct ?    0 Yes 5 No
Raghu
 
Answer
# 7
Take 3 testtubes and pour DNA,RNA and protines. Subjecte
these solutions to colory meter or spectro photo meter and
the obsrebence of these solutions will be different because
of DNA is double standard and it obserbs more light and it
give od 280 nano meters. coming to RNA, it gives more od
compare to DNA because of its a single standard. coming to
protines gives more od, because of it contains free amino
acids.

Finally these 3 solutions depends on the od.

another way to find density gradiant centrifigation of
these three molecules separated by their molecular weights.
 
Is This Answer Correct ?    2 Yes 7 No
Shilpa M
 

 
 
 
Other Bio Technology Interview Questions
 
  Question Asked @ Answers
 
i have done Msc Biotechnology from Chennai university in India.very shortly iam going to relocate to USA getting the dependant visa as my husband is going to usa. can i do job in USA   1
WHICH FORM OF PLASMID DNA travels quickest thro agarose gel? IGCAR 9
How to calculate protien percentage in lowry method??   2
What is the difference between SDS Page gel electrophoresis and native gel? BCIL 2
Name some human products that are now synthesized by bacteria?   1
Dubai is desert how it is possible it is becaming greenary?   2
What does a reduction in pesticides and soil erosion do to help the environment?   1
Which form of plasmid DNA moves quickest thro Agarose gels? IGCAR 6
may I get a good jobs nd a handsome salary after completing bsc in biotechnology nd how much salary I may accept???   7
what percentage of agarose is required to load 2kb dna   7
what is the future of bio informatics in india?   2
what is red biotechnology? Biotech 15
 
For more Bio Technology Interview Questions Click Here 
 
 
 
 
 


   
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