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Categories >> Sciences >> Biological Sciences >> Bio Technology
 
 


 

 
 Micro Biology interview questions  Micro Biology Interview Questions (628)
 Bio Technology interview questions  Bio Technology Interview Questions (218)
 Bio Chemistry interview questions  Bio Chemistry Interview Questions (412)
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 Bio Physics interview questions  Bio Physics Interview Questions (67)
 Biological Sciences AllOther interview questions  Biological Sciences AllOther Interview Questions (33)
Question
if there is solution , how will u estimate that it contains 
DNA only, not RNA? how we differentiate DNA ,RNA and 
Protein in solution without studying its structure?
 Question Submitted By :: Pritish
I also faced this Question!!     Rank Answer Posted By  
 
  Re: if there is solution , how will u estimate that it contains DNA only, not RNA? how we differentiate DNA ,RNA and Protein in solution without studying its structure?
Answer
# 1
Spectrophotometrically.OD at 260nm-DNA,280 for proteins 
Is This Answer Correct ?    44 Yes 5 No
Tk
 
  Re: if there is solution , how will u estimate that it contains DNA only, not RNA? how we differentiate DNA ,RNA and Protein in solution without studying its structure?
Answer
# 2
alkaline hydrolysis -rna is hydrolysed and become less
viscous while dna has no change .. when we shake a tube
containing pure proteins,it frothes... also takin OD at
260nm is a good option for distinguishing between dna and
protein .

pliz mail me if there is any mistake in this answer. thanx.
 
Is This Answer Correct ?    21 Yes 3 No
Jyothi
 
 
 
  Re: if there is solution , how will u estimate that it contains DNA only, not RNA? how we differentiate DNA ,RNA and Protein in solution without studying its structure?
Answer
# 3
just pour the solution in CsCl coloumn, and you will get
different bands. Also there is one very easy method to
detect protein solution from other two by just shaking. on
shaking solution containing protein will start foam
formation while those of DNA n RNA containing will not.
 
Is This Answer Correct ?    4 Yes 1 No
Abhinav Trivedi
 
  Re: if there is solution , how will u estimate that it contains DNA only, not RNA? how we differentiate DNA ,RNA and Protein in solution without studying its structure?
Answer
# 4
staining methods for DNA and RNA run on gel for DNA and RNA
for protein acylamide gel
 
Is This Answer Correct ?    0 Yes 5 No
Anuj
 
  Re: if there is solution , how will u estimate that it contains DNA only, not RNA? how we differentiate DNA ,RNA and Protein in solution without studying its structure?
Answer
# 5
use Nanodrop instrument 
Is This Answer Correct ?    0 Yes 5 No
Raghu
 
  Re: if there is solution , how will u estimate that it contains DNA only, not RNA? how we differentiate DNA ,RNA and Protein in solution without studying its structure?
Answer
# 6
value of od at240 / od at 260 = 0.8 for all nucleic acid. 
Is This Answer Correct ?    0 Yes 4 No
Aravind
 
  Re: if there is solution , how will u estimate that it contains DNA only, not RNA? how we differentiate DNA ,RNA and Protein in solution without studying its structure?
Answer
# 7
Take 3 testtubes and pour DNA,RNA and protines. Subjecte
these solutions to colory meter or spectro photo meter and
the obsrebence of these solutions will be different because
of DNA is double standard and it obserbs more light and it
give od 280 nano meters. coming to RNA, it gives more od
compare to DNA because of its a single standard. coming to
protines gives more od, because of it contains free amino
acids.

Finally these 3 solutions depends on the od.

another way to find density gradiant centrifigation of
these three molecules separated by their molecular weights.
 
Is This Answer Correct ?    2 Yes 7 No
Shilpa M
 

 
 
 
Other Bio Technology Interview Questions
 
  Question Asked @ Answers
 
If there were 4 bases for each code instead of the usual 3 bases, what would be the total number of possible codons? (its 64 when there r 3 bases for a codon) IGCAR 3
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Which hemeprotein helps to store oxygen in muscle tissues? IGCAR 3
what is the main objective of northern blotting?   6
 
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