Pyruvate is an intermediary organic acid metabolite in 
glycolysis and the first of the Embden Myerhoff pathway 
that can pass readily into or out of the cell. Thus, its 
addition to tissue culture medium provides both an energy 
source and a carbon skeleton for anabolic processes. Its 
addition may help in maintaining certain specialized cells, 
may help when cloning and may be necessary when the serum 
concentration is reduced in the medium (Culture of Animal 
Cells

Pyruvate repppuired for citric acid cycle or one of key 
intermediate in process of ATP formayion.


Napyruvate helps or works directly cell need noy to form it 
by de novo