What is the principle of HPLC?
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Answer / gopi
separation of mixture of compounds in a homogenous solution with an equilibration between two phases in which one is stationary phase and the other is mobile phase.
The principle is adsorption or partition based on the stationary phase used.
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How do we get end points and how many end points are possible for citric acid and di-acid not theorotically answer should be given practically.
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iam using ph instrument lab india make.3ponit and 5point.in calibration time 3point slope will come 98.89and 96.87.that limit is 85 to 105.what basis slope will come.any calucalation between them.5point wiicome slope 99.89?why 3p0int came 2 values
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