:: Home Page            
 Advertise your Business Here     
Browse  |   Placement Papers  |   Company  |   Code Snippets  |   Certifications  |   Visa Questions
Post Question  |   Post Answer  |   My Panel  |   Search  |   Articles  |   Topics  |   ERRORS new
   Refer this Site  Refer This Site to Your Friends  Site Map  Bookmark this Site  Set it as your HomePage  Contact Us     Login  |  Sign Up                      
Categories >> Sciences >> Biological Sciences >> Biological Sciences AllOther


 Micro Biology interview questions  Micro Biology Interview Questions (631)
 Bio Technology interview questions  Bio Technology Interview Questions (219)
 Bio Chemistry interview questions  Bio Chemistry Interview Questions (413)
 Bio Informatics interview questions  Bio Informatics Interview Questions (179)
 Genetics interview questions  Genetics Interview Questions (196)
 Botany interview questions  Botany Interview Questions (679)
 Zoology interview questions  Zoology Interview Questions (198)
 Cell Biology interview questions  Cell Biology Interview Questions (305)
 Bio Physics interview questions  Bio Physics Interview Questions (67)
 Biological Sciences AllOther interview questions  Biological Sciences AllOther Interview Questions (37)
Explain the Gas chromatography ?
 Question Submitted By :: Biological-Sciences-AllOther
I also faced this Question!!     Answer Posted By  
# 1
Gas chramatography consist of Gas solid chramatography(GLC)
and Gas liquied chramatography (GLS). In both cases Gas are
used as Mobile phase and either Liquieds are solieds are
act as Statinary phase.
GAS SOLID CHRAMATOGRAPHY :Gas solid chramatography(GLC)not
widely used in pharamacitricul industery because of limited
number of statinary phases are available.The separation
principle is the adsorption in some cases Gas solid
chramatography is used if the solute is not soluble in
stationary phase that situations Gas solid chramatography
GAS LIQUIED CHRAMATOGRAPHY:Gas liquied chramatography(GLS)
Gas are used as Mobile phase Liquieds are act as Statinary
phase.Liquieds are coated on the solid support in the form
layer.the layer should be 0.5 to 1.0u
In gas chramatography process the components are convarted
into vapour stage and mixed with gaseous mobile phase.the
compound which is more soluble in stationary phase travels
slower and elluted later if the solute less soluble in
stationary phase travels faster and elluted out first. no
compounds have similar partition coefficient for fixed
combination of stationary phase, Mobile phase and other
conditions. Hence the compounds are separated accourding to
the partition coefficient
Is This Answer Correct ?    12 Yes 4 No
# 2
GC may b gas solid chromatography or gas liquid
chromatography.In GSC stationary phase used is a solid and
mobile phase is carrier gas .the principle involved is
.Whereas in GLC stationary phase used is liquid coated onto
a solid support.and mobile phase is carrier gas .here the
principle involved is partition.based on partition
coefficent,the compound which is more soluble in mobile
phase elutes first and compound which is more soluble in
stationary phase elutes last.carrier gas used is
helium,nitrogen,or hydrogen.GC requires temperature
programming.columns used may be packed or capilllary columns
which may be open tubular columns(SCOT,WCOT).IT also
requires flow regulators,flow
metrs,preheater,detectors(electron capture,thermal
conductivity,flame photometric,flame ionisation,)
Is This Answer Correct ?    5 Yes 4 No

Other Biological Sciences AllOther Interview Questions
  Question Asked @ Answers
Describe the E. coli culture contamination ?   1
Give an account on Saccharomyces ?   2
What does CH2O represent?   2
What is the scope to ayurveda in future.   1
which soil requires least use of fertilizers Air-Force 6
When does rain fall?   2
Explain the Gas chromatography ?   2
How 70% alcohol works as disinfectant? Sugen 7
What is the importance of Chloramphenicol with Fungi ?   1
What is the function of RISUG (Reversible Inhibition of Sperm Under Guidance)?   1
How to use agar for antibiotic sensitivity testing ?   2
the amount of heat required will be minimum when a body is heated through-   1
For more Biological Sciences AllOther Interview Questions Click Here 

Copyright Policy  |  Terms of Service  |  Articles  |  Site Map  |  RSS Site Map  |  Contact Us
Copyright 2013  All Rights Reserved.   ::