IN POur plate technique the sample is serially diluted in
saline(9ml saline+iml sample-1st tube), from the first 1ml
is taken and poured into the 2nd tube.the selected dilution
is taken and from that 1ml is poured into the
petriplate.meanwhile the nutrient agar is prepared and
tempered at 45 degreecelcius,.it is poured on to the sample
and the plate is rotated slightly for uniform spreading of
sample in the agar.then the plates are incubated at 37
degree for 24 hours.subsurface colonies seen.anaerobic
in spread plate,it is done in similar way but the 0.1ml
sample is poured on the agar surface.with the l rod it is
evenly spreade and incubate at same temperature.aerobic
bacteria can grow.contamination is possible.both gives
pour plate: diluted microbial sample is transfered first to
the petriplate and over that melted medium is poured.highly
useful for enumerating the population of microbes in the
spread plate: melted medium is poured first and over that
add diluted microbial sample and spread using a sterile
bent glass rod. mainly for purification of a mixed bacteria
in spread plate method the mixed culture is not directly
poured into the culture medium,but it is made regularly
diluted into different testtube with the help of sterile
bent glass rod.now a sample is removed from each tube and
placed it into the agar medium.
In pour plate method the mixed culture is diluted directly
,in the cooled agar medium.the medium is maintained in a
cooled liquid state at a temperature of about 45*C.now the
let the medium be cooled so that agar agar medium can easily
get solidify.incubate it overanight .colonies may develop.
Spread plate technique is one of the most widely used methds for the isolation of microbes from their natural habitat.it is used for the sepretion of a dilute ,mixed with the population of microbes so that individual colonies can be isolate.this was performed by spreading 0.5ml of sample of culture on over the media that was used with a sterile L shaped glass rod while the petri plate spun is trunable.these cells will be deposited with bent glass rod on agar surfac.then incubate plates for suitable times then observed the plates.
in pour plate techniques the aerobic organisms may become dead when a hot media is poured over it and even if survives the oxygen conditions will not be sufficient for its production of colonies.. but this will not surely happen in spread plate technique as sterile medium is inoculated with sample.
It is the method of preparing TLC plates(Thin Layered
In pour plate method, the prepared stationary phase is just
poured on to the plate.
In spread plate method, the prepared stationary phase is
just poured & spread with a simple glass rod on to the plate.
Both these methods are out dated & are rarely used.