ALLInterview.com :: Home Page            
 Advertise your Business Here     
Browse  |   Placement Papers  |   Company  |   Code Snippets  |   Certifications  |   Visa Questions
Post Question  |   Post Answer  |   My Panel  |   Search  |   Articles  |   Topics  |   ERRORS new
   Refer this Site  Refer This Site to Your Friends  Site Map  Bookmark this Site  Set it as your HomePage  Contact Us     Login  |  Sign Up                      
Google
   
 
Categories >> Sciences >> Biological Sciences >> Micro Biology
 
 


 

 
 Micro Biology interview questions  Micro Biology Interview Questions (628)
 Bio Technology interview questions  Bio Technology Interview Questions (218)
 Bio Chemistry interview questions  Bio Chemistry Interview Questions (412)
 Bio Informatics interview questions  Bio Informatics Interview Questions (179)
 Genetics interview questions  Genetics Interview Questions (193)
 Botany interview questions  Botany Interview Questions (678)
 Zoology interview questions  Zoology Interview Questions (194)
 Cell Biology interview questions  Cell Biology Interview Questions (305)
 Bio Physics interview questions  Bio Physics Interview Questions (67)
 Biological Sciences AllOther interview questions  Biological Sciences AllOther Interview Questions (33)
Question
Explain Pour plate & spread plate ?
 Question Submitted By :: Guest
I also faced this Question!!     Rank Answer Posted By  
 
  Re: Explain Pour plate & spread plate ?
Answer
# 1
One of the advantages of pour plate is we can identify
whetheer bacteria is an anaerobe, aerobe or a facultative
aerobe.

In a pour plate, bacteria will trap in the agar and the
anaerobe and facultative anaerobe will grow within the
media.

For a spread plate, agar will have to be pour on top of the
solidified agar to create an anaerobic environment.


The pour plate is also less work . But contamination will
be a problem with spread plate and pour plate could help
in this issue.

One of the disadvantage of the pour plate is it will be
difficult when colonies are required to be picked for
further screening and analyses.

 
Is This Answer Correct ?    87 Yes 37 No
Swapna
 
  Re: Explain Pour plate & spread plate ?
Answer
# 2
the medium is under agar& on surface in spread 
Is This Answer Correct ?    11 Yes 53 No
Mafaza
 
 
 
  Re: Explain Pour plate & spread plate ?
Answer
# 3
pour plate: diluted microbial sample is transfered first to
the petriplate and over that melted medium is poured.highly
useful for enumerating the population of microbes in the
sample.
spread plate: melted medium is poured first and over that
add diluted microbial sample and spread using a sterile
bent glass rod. mainly for purification of a mixed bacteria
 
Is This Answer Correct ?    45 Yes 19 No
Anitha, K.g.
 
  Re: Explain Pour plate & spread plate ?
Answer
# 4
It is the method of preparing TLC plates(Thin Layered
Chromatographic plates)

In pour plate method, the prepared stationary phase is just
poured on to the plate.
In spread plate method, the prepared stationary phase is
just poured & spread with a simple glass rod on to the plate.

Both these methods are out dated & are rarely used.
 
Is This Answer Correct ?    4 Yes 34 No
Babu
 
  Re: Explain Pour plate & spread plate ?
Answer
# 5
IN POur plate technique the sample is serially diluted in
saline(9ml saline+iml sample-1st tube), from the first 1ml
is taken and poured into the 2nd tube.the selected dilution
is taken and from that 1ml is poured into the
petriplate.meanwhile the nutrient agar is prepared and
tempered at 45 degreecelcius,.it is poured on to the sample
and the plate is rotated slightly for uniform spreading of
sample in the agar.then the plates are incubated at 37
degree for 24 hours.subsurface colonies seen.anaerobic
bacteria seen.
in spread plate,it is done in similar way but the 0.1ml
sample is poured on the agar surface.with the l rod it is
evenly spreade and incubate at same temperature.aerobic
bacteria can grow.contamination is possible.both gives
viable count.
 
Is This Answer Correct ?    29 Yes 4 No
Kavitha
 
  Re: Explain Pour plate & spread plate ?
Answer
# 6
in spread plate method the mixed culture is not directly
poured into the culture medium,but it is made regularly
diluted into different testtube with the help of sterile
bent glass rod.now a sample is removed from each tube and
placed it into the agar medium.
In pour plate method the mixed culture is diluted directly
,in the cooled agar medium.the medium is maintained in a
cooled liquid state at a temperature of about 45*C.now the
let the medium be cooled so that agar agar medium can easily
get solidify.incubate it overanight .colonies may develop.
 
Is This Answer Correct ?    12 Yes 2 No
Vikram Bahadur Lal
 
  Re: Explain Pour plate & spread plate ?
Answer
# 7
Spread plate technique is one of the most widely used methds for the isolation of microbes from their natural habitat.it is used for the sepretion of a dilute ,mixed with the population of microbes so that individual colonies can be isolate.this was performed by spreading 0.5ml of sample of culture on over the media that was used with a sterile L shaped glass rod while the petri plate spun is trunable.these cells will be deposited with bent glass rod on agar surfac.then incubate plates for suitable times then observed the plates. 
Is This Answer Correct ?    5 Yes 3 No
Shuchi Upadhyay
 

 
 
 
Other Micro Biology Interview Questions
 
  Question Asked @ Answers
 
Which pathogen causes tetanus?   4
how are t cells differnt from b cells   6
What is the difference between gram positive and gram negative bacteria?   3
How to grow more E.coli?   1
What color is the microfungi penicillium?   4
ano po ba ang pinaka mabilis na ibon sa mundo   1
Describe the stages in the general life cycle of the flukes?   1
what is Sauton's media recipe?   2
what is global warming? cause of it? what is carbon credit? Banking 1
who is genious of indian microbiologist? Reddy-Labs 6
Explain the Plasmid DNA Purification ?   1
What is Cholera red reaction ?   1
 
For more Micro Biology Interview Questions Click Here 
 
 
 
 
 


   
Copyright Policy  |  Terms of Service  |  Articles  |  Site Map  |  RSS Site Map  |  Contact Us
   
Copyright 2013  ALLInterview.com.  All Rights Reserved.

ALLInterview.com   ::  KalAajKal.com