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Categories >> Sciences >> Biological Sciences >> Micro Biology
 
 


 

 
 Micro Biology interview questions  Micro Biology Interview Questions (631)
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Question
Explain Pour plate & spread plate ?
 Question Submitted By :: Micro-Biology
I also faced this Question!!     Answer Posted By  
 
Answer
# 1
One of the advantages of pour plate is we can identify
whetheer bacteria is an anaerobe, aerobe or a facultative
aerobe.

In a pour plate, bacteria will trap in the agar and the
anaerobe and facultative anaerobe will grow within the
media.

For a spread plate, agar will have to be pour on top of the
solidified agar to create an anaerobic environment.


The pour plate is also less work . But contamination will
be a problem with spread plate and pour plate could help
in this issue.

One of the disadvantage of the pour plate is it will be
difficult when colonies are required to be picked for
further screening and analyses.

 
Is This Answer Correct ?    93 Yes 39 No
Swapna
 
Answer
# 2
IN POur plate technique the sample is serially diluted in
saline(9ml saline+iml sample-1st tube), from the first 1ml
is taken and poured into the 2nd tube.the selected dilution
is taken and from that 1ml is poured into the
petriplate.meanwhile the nutrient agar is prepared and
tempered at 45 degreecelcius,.it is poured on to the sample
and the plate is rotated slightly for uniform spreading of
sample in the agar.then the plates are incubated at 37
degree for 24 hours.subsurface colonies seen.anaerobic
bacteria seen.
in spread plate,it is done in similar way but the 0.1ml
sample is poured on the agar surface.with the l rod it is
evenly spreade and incubate at same temperature.aerobic
bacteria can grow.contamination is possible.both gives
viable count.
 
Is This Answer Correct ?    31 Yes 4 No
Kavitha
 
 
 
Answer
# 3
pour plate: diluted microbial sample is transfered first to
the petriplate and over that melted medium is poured.highly
useful for enumerating the population of microbes in the
sample.
spread plate: melted medium is poured first and over that
add diluted microbial sample and spread using a sterile
bent glass rod. mainly for purification of a mixed bacteria
 
Is This Answer Correct ?    45 Yes 21 No
Anitha, K.g.
 
Answer
# 4
in spread plate method the mixed culture is not directly
poured into the culture medium,but it is made regularly
diluted into different testtube with the help of sterile
bent glass rod.now a sample is removed from each tube and
placed it into the agar medium.
In pour plate method the mixed culture is diluted directly
,in the cooled agar medium.the medium is maintained in a
cooled liquid state at a temperature of about 45*C.now the
let the medium be cooled so that agar agar medium can easily
get solidify.incubate it overanight .colonies may develop.
 
Is This Answer Correct ?    12 Yes 2 No
Vikram Bahadur Lal
 
Answer
# 5
Spread plate technique is one of the most widely used methds for the isolation of microbes from their natural habitat.it is used for the sepretion of a dilute ,mixed with the population of microbes so that individual colonies can be isolate.this was performed by spreading 0.5ml of sample of culture on over the media that was used with a sterile L shaped glass rod while the petri plate spun is trunable.these cells will be deposited with bent glass rod on agar surfac.then incubate plates for suitable times then observed the plates. 
Is This Answer Correct ?    6 Yes 3 No
Shuchi Upadhyay
 
Answer
# 6
One point of explanation could be this
In pour plate colonies/growth is found submerged in the
media whereas in spread plate the growth/colonies is found
on the surface.
 
Is This Answer Correct ?    1 Yes 0 No
Vinaya Salunkhe
 
Answer
# 7
in pour plate techniques the aerobic organisms may become dead when a hot media is poured over it and even if survives the oxygen conditions will not be sufficient for its production of colonies.. but this will not surely happen in spread plate technique as sterile medium is inoculated with sample. 
Is This Answer Correct ?    0 Yes 0 No
Vasantha
 
Answer
# 8
It is the method of preparing TLC plates(Thin Layered
Chromatographic plates)

In pour plate method, the prepared stationary phase is just
poured on to the plate.
In spread plate method, the prepared stationary phase is
just poured & spread with a simple glass rod on to the plate.

Both these methods are out dated & are rarely used.
 
Is This Answer Correct ?    4 Yes 37 No
Babu
 
Answer
# 9
the medium is under agar& on surface in spread 
Is This Answer Correct ?    11 Yes 56 No
Mafaza
 

 
 
 
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For more Micro Biology Interview Questions Click Here 
 
 
 
 
 


   
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