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Categories >> Sciences >> Chemistry >> Analytical Chemistry
 
 


 

 
 Organic Chemistry interview questions  Organic Chemistry Interview Questions (228)
 Inorganic Chemistry interview questions  Inorganic Chemistry Interview Questions (96)
 Analytical Chemistry interview questions  Analytical Chemistry Interview Questions (867)
 Physical Chemistry interview questions  Physical Chemistry Interview Questions (57)
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 Chemistry AllOther interview questions  Chemistry AllOther Interview Questions (71)
Question
what is the difference between gc& hplc. why we r not
calculating purity by gc for final products
 Question Submitted By :: Analytical-Chemistry
I also faced this Question!!     Answer Posted By  
 
Answer
# 1
Gas chromatography (GC) is based on a partition equilibrium
of analyte between a solid stationary phase (often a liquid
silicone-based material) and a mobile gas (most often Helium).

In the HPLC technique, the sample is forced through a column
that is packed with a stationary phase composed of
irregularly or spherically shaped particles, a porous
monolithic layer, or a porous membrane by a liquid (mobile
phase) at high pressure

GC is used for volatile compounds (those that break down
rapidly) while HPLC is better for less volatile samples. If
a sample contains salts or carries a charge, it must be
analyzed using HPLC, not GC

GC is used for volatile compounds (those that break down
rapidly) while HPLC is better for less volatile samples. If
a sample contains salts or carries a charge, it must be
analyzed using HPLC, not GC
 
Is This Answer Correct ?    18 Yes 3 No
Dhanalakshmi
 
Answer
# 2
In GC mobile phase is Gas and in HPLC is liquid.
due to its resolution power.
 
Is This Answer Correct ?    7 Yes 5 No
D.lodhiya
 
 
 
Answer
# 3
 
Is This Answer Correct ?    0 Yes 0 No
ANALYTICALSTUDENT
 
Answer
# 4
In the majority of the cases the analytical method of
choices is either GC or HPLC. GC generally selected where
the compounds to be analysed are volatile, of low molecular
weight, thermally stable and non polar.In HPLC methods
developed for analytical work with picograms, nanograms of
compounds are simply transferred to larger columns to higher
flow rates for preparative chromatography for the isolation
of milligrams or grams of compounds. Similar scale
preparative GC would be impractical, if not impossible, in
the laboratory.
 
Is This Answer Correct ?    1 Yes 1 No
Nidhi Srivastava
 
Answer
# 5
GC is used for volatile compounds & HPLC is used for
non-volatile compounds. And because of in your product may
be non-volatile impurities.So, not calculating purity by gc
for final products.
 
Is This Answer Correct ?    0 Yes 1 No
Nirav
 

 
 
 
Other Analytical Chemistry Interview Questions
 
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why KBr is used in FTIR SPECTROSCOPY and why is it necessary to triturate KBr during FTIR analysis?   14
Why sodium lamp is used in polarimeter? Aurobindo 1
what is the conductivity of hplc grade water?   3
acceptance criteria for ph meter, hplc detector,pump,injector,oven when we calibrate.   3
How will decide to use HPLC OR GC for developing method for unkown compund   5
Which buffer is used for LC/MS mobile phase & at What Conc.   3
WHAT ARE THE LIMITS FOR UNIFORMITY DOSAGE CONTENT FOR TABLETS AND CAPSULES Micro-Labs 3
How can the GC or HPLC method is selected to determine the impurity profile in drug product? Ajanta 1
what is a differance between assay by HPLC and assay by titration?   4
what is the difference between mcg and ppm?   4
what is mean by content uniformity?   2
what is mean by 21 CFR PART? In that what is 21 ?   1
 
For more Analytical Chemistry Interview Questions Click Here 
 
 
 
 
 


   
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