ALLInterview.com :: Home Page            
 Advertise your Business Here     
Browse  |   Placement Papers  |   Company  |   Code Snippets  |   Certifications  |   Visa Questions
Post Question  |   Post Answer  |   My Panel  |   Search  |   Articles  |   Topics  |   ERRORS new
   Refer this Site  Refer This Site to Your Friends  Site Map  Bookmark this Site  Set it as your HomePage  Contact Us     Login  |  Sign Up                      
Google
   
 
Categories >> Sciences >> Chemistry >> Analytical Chemistry
 
 


 

 
 Organic Chemistry interview questions  Organic Chemistry Interview Questions (221)
 Inorganic Chemistry interview questions  Inorganic Chemistry Interview Questions (95)
 Analytical Chemistry interview questions  Analytical Chemistry Interview Questions (833)
 Physical Chemistry interview questions  Physical Chemistry Interview Questions (57)
 General Chemistry interview questions  General Chemistry Interview Questions (282)
 Chemistry AllOther interview questions  Chemistry AllOther Interview Questions (65)
Question
what is the difference between gc& hplc. why we r not
calculating purity by gc for final products
 Question Submitted By :: Raju
I also faced this Question!!     Rank Answer Posted By  
 
  Re: what is the difference between gc& hplc. why we r not calculating purity by gc for final products
Answer
# 1
Gas chromatography (GC) is based on a partition equilibrium
of analyte between a solid stationary phase (often a liquid
silicone-based material) and a mobile gas (most often Helium).

In the HPLC technique, the sample is forced through a column
that is packed with a stationary phase composed of
irregularly or spherically shaped particles, a porous
monolithic layer, or a porous membrane by a liquid (mobile
phase) at high pressure

GC is used for volatile compounds (those that break down
rapidly) while HPLC is better for less volatile samples. If
a sample contains salts or carries a charge, it must be
analyzed using HPLC, not GC

GC is used for volatile compounds (those that break down
rapidly) while HPLC is better for less volatile samples. If
a sample contains salts or carries a charge, it must be
analyzed using HPLC, not GC
 
Is This Answer Correct ?    17 Yes 3 No
Dhanalakshmi
 
  Re: what is the difference between gc& hplc. why we r not calculating purity by gc for final products
Answer
# 2
In GC mobile phase is Gas and in HPLC is liquid.
due to its resolution power.
 
Is This Answer Correct ?    7 Yes 5 No
D.lodhiya
 
 
 
  Re: what is the difference between gc& hplc. why we r not calculating purity by gc for final products
Answer
# 3
 
Is This Answer Correct ?    0 Yes 0 No
ANALYTICALSTUDENT
[DRREDDY LAB]
 
  Re: what is the difference between gc& hplc. why we r not calculating purity by gc for final products
Answer
# 4
GC is used for volatile compounds & HPLC is used for
non-volatile compounds. And because of in your product may
be non-volatile impurities.So, not calculating purity by gc
for final products.
 
Is This Answer Correct ?    0 Yes 1 No
Nirav
 
  Re: what is the difference between gc& hplc. why we r not calculating purity by gc for final products
Answer
# 5
In the majority of the cases the analytical method of
choices is either GC or HPLC. GC generally selected where
the compounds to be analysed are volatile, of low molecular
weight, thermally stable and non polar.In HPLC methods
developed for analytical work with picograms, nanograms of
compounds are simply transferred to larger columns to higher
flow rates for preparative chromatography for the isolation
of milligrams or grams of compounds. Similar scale
preparative GC would be impractical, if not impossible, in
the laboratory.
 
Is This Answer Correct ?    1 Yes 1 No
Nidhi Srivastava
 

 
 
 
Other Analytical Chemistry Interview Questions
 
  Question Asked @ Answers
 
how do you perform reverse enginearing study for drug containing sodium chloride sodium citrate sodium bicarbonate Strides 1
what is the moisture content of Disodium hydrogen tartrate. how it can be calculted. as 16.5%   1
1)why use benzophenon,uracil in hplc calibration. 2)why use sucrose in polarimeter calibration. 3)what is S/N ratio, noise and drift in hplc 4)how we can find lod and loq in method validation   1
which solvent is highest poler acetonitrile dimethyl formamide methanole tetrahydrofurane buffer solution   8
why r u using hplc grade solvents   3
What is blank titration of potentiometrically.   2
how can you do the analysis of compound given to u by hplc   1
How we develop a method by HPLC for unknown compound,if we got three peaks with same response then how we can find out which one is our desired compound's peak without taking help of LC MS. Kemwell 1
principle of FTIR Macleods 2
state the principle of gas chromatography?   4
Why we use caffeine for HPLC calibration in Detector wave ength accuracy test Cipla 53
what is the diff betwen calibration and system suitability?   7
 
For more Analytical Chemistry Interview Questions Click Here 
 
 
 
 
 


   
Copyright Policy  |  Terms of Service  |  Articles  |  Site Map  |  RSS Site Map  |  Contact Us
   
Copyright 2013  ALLInterview.com.  All Rights Reserved.

ALLInterview.com   ::  KalAajKal.com