In RFLP analysis, the DNA of an organism is cut up into 
fragments using restriction enzymes. A large number of 
short fragments of DNA will be produced.

Restriction enzymes always cut at the same base sequence.


Because no two individuals have identical DNA, no two 
individuals will have the same length fragments. For 
example, the enzyme EcoRI always cuts DNA at the sequence 
GAATTC. 

Different people  have different numbers of this particular 
sequence and will therefore have different fragment 
lengths.  some of them will be at different locations on 
the chromosome.

DNA were placed on the gelatin.

The DNA bands must be stained to make them visible.

Ethidium bromide-stained DNA will fluoresce when 
illuminated with UV light.

PCR techniques are used to produce sufficient quantities of 
DNA for this technique.