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Categories >> Sciences >> Chemistry >> Analytical Chemistry
 
 


 

 
 Organic Chemistry interview questions  Organic Chemistry Interview Questions (222)
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 Analytical Chemistry interview questions  Analytical Chemistry Interview Questions (848)
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Question
What is the difference between Normal phase method & 
Reverse method in HPLC ? Define application of both method.
 Question Submitted By :: Analytical-Chemistry
I also faced this Question!!     Answer Posted By  
 
Answer
# 1
Normal phase: Stationary phase is polar, mobile phase is non
polar,
Reverse phase: Stationary phase is non polar, mobile phase is
polar.
 
Is This Answer Correct ?    35 Yes 1 No
K L Srinivas
 
Answer
# 2
Normal phase: Stationary phase is polar, mobile phase is non
polar,
Reverse phase: Stationary phase is non polar, mobile phase is
polar.

normal phase is based on the adsorption phenomenon and
reverse phase is based on the partition phenomenon

normal phase used for chiral compound and reverse phase used
for other
 
Is This Answer Correct ?    13 Yes 1 No
Maulik
 
 
 
Answer
# 3
above answer is perfect and also,,,....

Normal Phase: separates analytes on the basis of their
affinity for a polar stationary surface such as silica.

Reversed Phase: separates on the basis hydrophobic
interacertions, which originates from the high symmetry in
the dipolar water structure
 
Is This Answer Correct ?    2 Yes 0 No
Srikanth
 

 
 
 
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