ALLInterview.com :: Home Page            
 Advertise your Business Here     
Browse  |   Placement Papers  |   Company  |   Code Snippets  |   Certifications  |   Visa Questions
Post Question  |   Post Answer  |   My Panel  |   Search  |   Articles  |   Topics  |   ERRORS new
   Refer this Site  Refer This Site to Your Friends  Site Map  Bookmark this Site  Set it as your HomePage  Contact Us     Login  |  Sign Up                      
Google
   
 
Categories >> Sciences >> Chemistry >> Analytical Chemistry
 
 


 

 
 Organic Chemistry interview questions  Organic Chemistry Interview Questions (228)
 Inorganic Chemistry interview questions  Inorganic Chemistry Interview Questions (96)
 Analytical Chemistry interview questions  Analytical Chemistry Interview Questions (867)
 Physical Chemistry interview questions  Physical Chemistry Interview Questions (57)
 General Chemistry interview questions  General Chemistry Interview Questions (284)
 Chemistry AllOther interview questions  Chemistry AllOther Interview Questions (71)
Question
What is the difference between Normal phase method & 
Reverse method in HPLC ? Define application of both method.
 Question Submitted By :: Analytical-Chemistry
I also faced this Question!!     Answer Posted By  
 
Answer
# 1
Normal phase: Stationary phase is polar, mobile phase is non
polar,
Reverse phase: Stationary phase is non polar, mobile phase is
polar.
 
Is This Answer Correct ?    36 Yes 1 No
K L Srinivas
 
Answer
# 2
Normal phase: Stationary phase is polar, mobile phase is non
polar,
Reverse phase: Stationary phase is non polar, mobile phase is
polar.

normal phase is based on the adsorption phenomenon and
reverse phase is based on the partition phenomenon

normal phase used for chiral compound and reverse phase used
for other
 
Is This Answer Correct ?    13 Yes 1 No
Maulik
 
 
 
Answer
# 3
above answer is perfect and also,,,....

Normal Phase: separates analytes on the basis of their
affinity for a polar stationary surface such as silica.

Reversed Phase: separates on the basis hydrophobic
interacertions, which originates from the high symmetry in
the dipolar water structure
 
Is This Answer Correct ?    2 Yes 0 No
Srikanth
 

 
 
 
Other Analytical Chemistry Interview Questions
 
  Question Asked @ Answers
 
WHY KBR IS USED IN IR FOR PREPARATION OF PELLETE,WHY IT SHOWS ZERO DIPOLEMOMENT GVK 2
How you will select column for HPLC development depends on nature of compound and how it will work explain in brief?   3
what is the significant of pH in HPlc? Orchid 4
Why forced Digradition Study is done   3
In Stability testing if significant change occurs then what will be the action plan? Cipla 2
how much you expected salary? GSK-GlaxoSmithKline 7
Why we are performing wavelength accuracy at lower and higher wavelengths in HPLC calibration   3
What is difference between Chromatographic purity and Related substances analysis?   2
what is dissolution   5
what is the difference between LOSS ON DRYING IN ORDINARY OVEN TECHNIQUE AND VACUUM OVEN TECHNIQUE Divis-Laboratories 3
What is the SOP for SOP JBCPL 4
1 % solution is equal to how much ppm   28
 
For more Analytical Chemistry Interview Questions Click Here 
 
 
 
 
 


   
Copyright Policy  |  Terms of Service  |  Articles  |  Site Map  |  RSS Site Map  |  Contact Us
   
Copyright 2013  ALLInterview.com.  All Rights Reserved.

ALLInterview.com   ::  KalAajKal.com