What is the procedure for UV-spectrophotometer calibration?

Answers were Sorted based on User's Feedback



What is the procedure for UV-spectrophotometer calibration?..

Answer / manikatna

UV-VIS spectrophotometer calibration is many parameters
which is
1)control of wavelength
2)control of absorbance
3)wavelength accuracy
4)straylight accuracy
5)resolution
6)resolution power

Is This Answer Correct ?    172 Yes 15 No

What is the procedure for UV-spectrophotometer calibration?..

Answer / rakesh

1.0 CONTROL OF ABSORBANCES
PROCEDURE: REPARATION OF POTASSIUM DICROMATE SOLUTION: Dry
K2Cr2O7 at 130oC for 2 hours. Weigh accurately about the
quantity of sample equal to 60mg into 1000ml volumetric
flask. Add 0.005m Sulphuric acid to dissolve and dilute
with the same solvent.
Find out the absorbances of (A 1% 1cm) above said solution
at wavelengths of a 235nm, 257nm, 313nm and 350nm.
Calculate the (1% 1cm) value using the following expression.
Absorbance X 1 X 1000 / Weight of K2Cr2O7 in g X 100
Check the value for each wavelength with the tolerances
given four wavelengths.


PREPARATION OF REAGENT:
(i) Dilute 3ml of concentrated sulphuric acid and make up
100ml with distilled water.
(ii) Dilute 20ml to 2000ml with distilled water.
2. LIMIT OF STRAYLIGHT:
The absorbance of a 1.2-% w/v solution of potassium
chloride at a path-length of 1cm should be greater than 2.0
at about 200nm when compared with water as reference liquid.
1.2% POTASSIUM CHLORIDE SOLUTION:
Take 1.2 g of Potassium chloride previously dried at 105oC
for two hours into the 100ml volumetric flask. Add water to
dissolve and make up to volume with water.
3. RESOLUTION POWER:
Record the spectrum of a 0.02% v/v solution of Toluene in
Hexane UV. The ratio of the absorbance at the maximum at
about 269nm to that at the minimum at about 266nm is not
less than 1.5 unless other wise specified in the monograph.
0.02% v/v TOLUENE:
Take 0.02ml of Toluene into the 100ml volumetric flask. Add
Hexane to volume.

Is This Answer Correct ?    97 Yes 8 No

What is the procedure for UV-spectrophotometer calibration?..

Answer / upk

UV-VIS spectrophotometer calibration is many parameters
which is
1)control of wavelength
2)control of absorbance
3)wavelength accuracy
4)straylight accuracy
5)resolution
6)resolution power &
7) Io Line Flatnes test

Is This Answer Correct ?    55 Yes 22 No

What is the procedure for UV-spectrophotometer calibration?..

Answer / nitesh pal

in above answer also added 430 nm in control of wavelength.(acc to ipm dilution is about 60 mg to 1ooml (same solvent)

Is This Answer Correct ?    21 Yes 8 No

What is the procedure for UV-spectrophotometer calibration?..

Answer / ramkrishna jagtap

control of wavelength

Is This Answer Correct ?    7 Yes 0 No

What is the procedure for UV-spectrophotometer calibration?..

Answer / devender justa

Internal Calibration ,external Calibration, Baseline
Flatness

Is This Answer Correct ?    8 Yes 4 No

Post New Answer

More Analytical Chemistry Interview Questions

why we use glass fiber filters use in some situation?

0 Answers  


what is the differnce between GMP and CGMP

7 Answers   Shilpa Medicare,


what is titrent,& titrate

2 Answers   Graviti Pharmaceuticals,


what are the guidelines for analytical method validations?

0 Answers  


how we can identify the impurity is coming below loq at transfering site?

0 Answers  






key components in gas chromotagraphy.

1 Answers   Laurus,


What is definition of RRT and what is the difference between RT and RRT in HPLC

2 Answers  


In HPLC Calibration, On which basis RSD Limit of noise test is fixed (NMT 33.0 % )

0 Answers   Alembic,


How to do spike of aflatoxin in peanut to get maximum recovery

0 Answers  


why use k2cr2o7 , kcl h2so4 in uv calibration

1 Answers   DRL, GVK,


In gas chromatographic analysis sometimes we use headspace, why it is required instead of manually injection?

1 Answers  


What is related substance by HPLC impurity limits as per USP?

0 Answers  


Categories
  • Organic Chemistry Interview Questions Organic Chemistry (342)
  • Inorganic Chemistry Interview Questions Inorganic Chemistry (123)
  • Analytical Chemistry Interview Questions Analytical Chemistry (1387)
  • Physical Chemistry Interview Questions Physical Chemistry (62)
  • General Chemistry Interview Questions General Chemistry (478)
  • Chemistry AllOther Interview Questions Chemistry AllOther (190)