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Categories >> Sciences >> Chemistry >> Analytical Chemistry
 
 


 

 
 Organic Chemistry interview questions  Organic Chemistry Interview Questions (227)
 Inorganic Chemistry interview questions  Inorganic Chemistry Interview Questions (96)
 Analytical Chemistry interview questions  Analytical Chemistry Interview Questions (860)
 Physical Chemistry interview questions  Physical Chemistry Interview Questions (57)
 General Chemistry interview questions  General Chemistry Interview Questions (283)
 Chemistry AllOther interview questions  Chemistry AllOther Interview Questions (71)
Question
What is the procedure for UV-spectrophotometer calibration?
 Question Submitted By :: Analytical-Chemistry
I also faced this Question!!     Answer Posted By  
 
Answer
# 1
UV-VIS spectrophotometer calibration is many parameters
which is
1)control of wavelength
2)control of absorbance
3)wavelength accuracy
4)straylight accuracy
5)resolution
6)resolution power
 
Is This Answer Correct ?    92 Yes 8 No
Manikatna
 
Answer
# 2
1.0 CONTROL OF ABSORBANCES
PROCEDURE: REPARATION OF POTASSIUM DICROMATE SOLUTION: Dry
K2Cr2O7 at 130oC for 2 hours. Weigh accurately about the
quantity of sample equal to 60mg into 1000ml volumetric
flask. Add 0.005m Sulphuric acid to dissolve and dilute
with the same solvent.
Find out the absorbances of (A 1% 1cm) above said solution
at wavelengths of a 235nm, 257nm, 313nm and 350nm.
Calculate the (1% 1cm) value using the following expression.
Absorbance X 1 X 1000 / Weight of K2Cr2O7 in g X 100
Check the value for each wavelength with the tolerances
given four wavelengths.


PREPARATION OF REAGENT:
(i) Dilute 3ml of concentrated sulphuric acid and make up
100ml with distilled water.
(ii) Dilute 20ml to 2000ml with distilled water.
2. LIMIT OF STRAYLIGHT:
The absorbance of a 1.2-% w/v solution of potassium
chloride at a path-length of 1cm should be greater than 2.0
at about 200nm when compared with water as reference liquid.
1.2% POTASSIUM CHLORIDE SOLUTION:
Take 1.2 g of Potassium chloride previously dried at 105oC
for two hours into the 100ml volumetric flask. Add water to
dissolve and make up to volume with water.
3. RESOLUTION POWER:
Record the spectrum of a 0.02% v/v solution of Toluene in
Hexane UV. The ratio of the absorbance at the maximum at
about 269nm to that at the minimum at about 266nm is not
less than 1.5 unless other wise specified in the monograph.
0.02% v/v TOLUENE:
Take 0.02ml of Toluene into the 100ml volumetric flask. Add
Hexane to volume.
 
Is This Answer Correct ?    57 Yes 4 No
Rakesh
 
 
 
Answer
# 3
UV-VIS spectrophotometer calibration is many parameters
which is
1)control of wavelength
2)control of absorbance
3)wavelength accuracy
4)straylight accuracy
5)resolution
6)resolution power &
7) Io Line Flatnes test
 
Is This Answer Correct ?    39 Yes 15 No
Upk
 
Answer
# 4
in above answer also added 430 nm in control of wavelength.(acc to ipm dilution is about 60 mg to 1ooml (same solvent) 
Is This Answer Correct ?    12 Yes 2 No
Nitesh Pal
 
Answer
# 5
Internal Calibration ,external Calibration, Baseline
Flatness
 
Is This Answer Correct ?    2 Yes 1 No
Devender Justa
 

 
 
 
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