Top Bio Technology Interview Questions :: ALLInterview.com http://www.allinterview.com Top Bio Technology Interview Questions en-us how do mutations occur? http://www.allinterview.com/showanswers/21016.html 1. Mutation in genes can occur by chance in nature(Random Mutation) 2. Mutation can be induced in genes by chemicals in lab what is red biotechnology? http://www.allinterview.com/showanswers/10825.html Red biotechnology is biotechnology applied to medical processes. Some examples are the designing of organisms to produce antibiotics, and the engineering of genetic cures to diseases through genomic manipulation. Difference between Totipotent and pluripotent stem cells? http://www.allinterview.com/showanswers/6405.html Differences are very few between totipotent and pluripotent stem cells.The genetic code remains the same. The potency for making all bodily tissues remains the same. When using the blastocyst as source, the trophechtoderm is removed and this What is isotopic tracer technique? http://www.allinterview.com/showanswers/1668.html An isotopic tracer is used in chemistry and biochemistry to help understand chemical reactions and interactions. In this technique, one or more of the atoms of the molecule of interest is substituted for an atom of the same chemical element, I've an interview for post of Lecturer in a Biotechnology Depar http://www.allinterview.com/showanswers/32744.html What is Genetic Engineering? What are the differences between Genetic Engineering and breeding? Does Genetic Engineering harm or benefit human society? What are the down sides of Genetic Engineering? Can you name a few potential risks of Geneti State two advantages of using immobilised enzymes. http://www.allinterview.com/showanswers/16767.html 1) Better availability of active sites to the substrate molecules, due to the fixed position of the enzymes. 2) Wear and tear of the enzymes due to shear forces acting in the dissolved state on the enzyme. What is a Living Modified Organism (LMO)? http://www.allinterview.com/showanswers/16774.html Living modified organism, identical with GMO, means any living organism that possesses a novel combination of genetic material obtained through the use of modern biotechnology. It can be any biological entity capable of transferring or re What is the superiority of TLC over paper chromatography? http://www.allinterview.com/showanswers/1671.html The superiority of TLC over paper chromatography because of inorganic nature of adsorbent concentrated sulfuric acid spray followed by heating may be used to develope on the chromatogram by charring. moreover aminoacid mixtures which require 18 How can one separate prokaryotic m-RNA http://www.allinterview.com/showanswers/17211.html Separate the nucleoid (portion containing nucleic acids) of the prokaryote by cell lysis and differential centrifugation. After that, treat the extract with DNAse to remove any trace of DNA along with the RNA. The RNA thus obtained is mRNA. What is the basic difference between quantitative and quantal assay http://www.allinterview.com/showanswers/98.html Quantitative assays measure the actual number of infectious particles in the inoculum,while quantal assays only indicate the presence or absence of infectious viruses. What is the difference between free boundary electrophoresis and zon http://www.allinterview.com/showanswers/1670.html HERE in free electrophoresis protein solution is composed of 3 components a,b,c carry charges of same sign but of diferent magnitude. the solution will be homogeneous from ascending boundary to descending boundary. on the flow ofcurrent 3 compone What is meant by transversion? http://www.allinterview.com/showanswers/91.html Substitution of purine for a pyrimidine and vice versa in base pairing. Explain Gram Staining method? http://www.allinterview.com/showanswers/51.html 1) Fix smear by heat. 2) Cover with crystal violet. 3) Wash with water 4) Cover with gram’s iodine 5) Wash with water. do not blot. 6) Decolorize for 10-30 sec with gentle agitation in acetone(30ml)and alcohol(70ml). 7) Wash with water. do Name the bacterium from which the Bt gene is isolated. http://www.allinterview.com/showanswers/16771.html Bacillus thuringiensis What are introns and exons? http://www.allinterview.com/showanswers/64.html Dna insertions that generally are not found in prokaryotic genes are introns and several stretches of dna that do not function as codons occur between the coding sequences of genes. The coding regions of eukaryotic genes are exons.