Some proteins profoundly alter the structure of DNA when
they bind, bending it far out of its standard B
configuration. Where do you think the energy comes from to
distort the DNA?
Many genes are regulated in both a negative and a positive
manner. The genes mediating lactose metabolism in bacteria
are a classical example. What are the two small molecule
ligands that control expression of these genes? What does
each one do?
Review your data from the entire module. Say we ask you to
redo the purification.Is there any step that you could
eliminate? Which one? Why can you eliminate it?
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