Suppose you used the NCBI Blast server to look for a match
to a fruit-fly gene.
Suppose further that the best alignment contained this
section of an alignment:
Hit from database: XXXXXXXXXXXXXXNFSTSQ
user sequence: SLEAEAAPASISPSNFSSSQ
What do the Xs signify?
Which of the following sequences contains the pattern [AG]-x
(4)-G-K-[ST] from the PROSITE database?
seq. A: VAGWGKST
seq B: GVLKRGKS
seq. C: AGVLKGRT
seq. D: AGVGKSTP
Bootstrap analysis evaluates evolutionary trees by sampling
columns from the original alignment with replacement
(multiplying or removing some of them) and computing a
proportion of times that a particular branch appears in the
resulting trees. What is the main idea behind this approach?
How many base pairs does the simplest bacteria have?
45
what is a qi number?
62
What is enzyme-ligand interaction?
103
How to find a sequence of dna molecule?
60
What is a homologue?
71
Suppose the Blast search returned 100 hits. Of these, 17
were false positives and we knew that there were 165
sequences in the database which should have returned a hit
with our sequence. How many false negatives were there, and
what is the sensitivity and selectivity of Blast in this
instance?
If a match from a sequence database search is reported to
have an E-value of 0.0, should it be considered highly
insignificant or highly significant?
73
An electron is injected into a region of uniform magnetic
flux density with the components
of velocity parallel to and normal to the flux. What is the
path of the electron?
A certain radioactive element A, has a half life = t
seconds. In (t/2) seconds the fraction of the initial
quantity of the element so far decayed is nearly how much?
103
What are the current challenges in the Pharmaceutical and
biotechnology sectors?
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